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ser 536  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology ser 536
    Ser 536, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 775 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ser+536/pm41494249-67-0-34?v=Santa+Cruz+Biotechnology
    Average 96 stars, based on 775 article reviews
    ser 536 - by Bioz Stars, 2026-08
    96/100 stars

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    Image Search Results


    p65 translocation is not inhibited by CERT knockdown. A: Mechanism of NF-κB pathway activation. B: HASTR/ci35 cells were transfected with control siRNA (siCTRL) or siRNA against CERT (siCERT) and incubated for 48 h. TNF receptor 1 protein expression levels were analyzed by western blotting (n = 3). C: The cells were further incubated with 3 ng/ml IL-1α and 50 ng/ml TNF-α for 15 min to 6 h. The protein levels of IκBα and phospho-IκBα (p-IκBα) were examined by western blotting (n = 3). D: The cells were further incubated with 3 ng/ml IL-1α and 50 ng/ml TNF-α for 15 min. The cells were fixed and immunostained for p65. Typical images were shown (n = 3). B, Student’s t test. C, Tukey’s test. All data are presented as mean ± SEM. ns, not significant.

    Journal: Journal of Lipid Research

    Article Title: Sphingomyelin regulates astrocyte activity by regulating NF-κB signaling via HDAC1/3 expression

    doi: 10.1016/j.jlr.2025.100933

    Figure Lengend Snippet: p65 translocation is not inhibited by CERT knockdown. A: Mechanism of NF-κB pathway activation. B: HASTR/ci35 cells were transfected with control siRNA (siCTRL) or siRNA against CERT (siCERT) and incubated for 48 h. TNF receptor 1 protein expression levels were analyzed by western blotting (n = 3). C: The cells were further incubated with 3 ng/ml IL-1α and 50 ng/ml TNF-α for 15 min to 6 h. The protein levels of IκBα and phospho-IκBα (p-IκBα) were examined by western blotting (n = 3). D: The cells were further incubated with 3 ng/ml IL-1α and 50 ng/ml TNF-α for 15 min. The cells were fixed and immunostained for p65. Typical images were shown (n = 3). B, Student’s t test. C, Tukey’s test. All data are presented as mean ± SEM. ns, not significant.

    Article Snippet: Antibodies to p65 antibody (Cat# sc-8008), phospho-Ser 536 -p65 (Cat# sc-136548), TNF receptor 1 (TNFR1; Cat#sc-9436), histone deacetylase 1 (HDAC1; Cat# sc-81598), HDAC3 (Cat# sc-376957), calnexin (Cat# sc-46669), lamin B1 (Cat# sc-374015), and flotillin-1 (Cat# 74566) were obtained from Santa Cruz Biotechnology.

    Techniques: Translocation Assay, Knockdown, Activation Assay, Transfection, Control, Incubation, Expressing, Western Blot

    CERT knockdown reduces p65 acetylation. A: HASTR/ci35 cells were transfected with p65-FLAG plasmids. After 3 h, the cells were washed out and transfected with control siRNA (siCTRL) or siRNA against CERT (siCERT) and cultured for 48 h. The cells were further incubated with 3 ng/ml IL-1α and 50 ng/ml TNF-α for 30 min to 3 h. The protein levels of p65-FLAG and acetylated p65 were analyzed by western blotting (n = 3). B: HASTR/ci35 cells were transfected with control siRNA or siRNA against CERT and incubated for 48 h (n = 3). The protein levels of HDAC1 and HDAC3 were examined by western blotting (n = 3∼6). C: The cells were further pre-incubated with 3 mM valproic acid (VPA) for 30 min and then treated with 3 ng/ml IL-1α and 50 ng/ml TNF-α for 24 h. The mRNA levels of IL-6 , C3 , and COX-2 were quantified by qPCR (n = 4∼6). All data are presented as mean ± SEM. A and C, Tukey’s test. B, Student’s t test. ∗ P < 0.05 ∗∗ P < 0.01 ∗∗∗ P < 0.001 ∗∗∗∗ P < 0.0001.

    Journal: Journal of Lipid Research

    Article Title: Sphingomyelin regulates astrocyte activity by regulating NF-κB signaling via HDAC1/3 expression

    doi: 10.1016/j.jlr.2025.100933

    Figure Lengend Snippet: CERT knockdown reduces p65 acetylation. A: HASTR/ci35 cells were transfected with p65-FLAG plasmids. After 3 h, the cells were washed out and transfected with control siRNA (siCTRL) or siRNA against CERT (siCERT) and cultured for 48 h. The cells were further incubated with 3 ng/ml IL-1α and 50 ng/ml TNF-α for 30 min to 3 h. The protein levels of p65-FLAG and acetylated p65 were analyzed by western blotting (n = 3). B: HASTR/ci35 cells were transfected with control siRNA or siRNA against CERT and incubated for 48 h (n = 3). The protein levels of HDAC1 and HDAC3 were examined by western blotting (n = 3∼6). C: The cells were further pre-incubated with 3 mM valproic acid (VPA) for 30 min and then treated with 3 ng/ml IL-1α and 50 ng/ml TNF-α for 24 h. The mRNA levels of IL-6 , C3 , and COX-2 were quantified by qPCR (n = 4∼6). All data are presented as mean ± SEM. A and C, Tukey’s test. B, Student’s t test. ∗ P < 0.05 ∗∗ P < 0.01 ∗∗∗ P < 0.001 ∗∗∗∗ P < 0.0001.

    Article Snippet: Antibodies to p65 antibody (Cat# sc-8008), phospho-Ser 536 -p65 (Cat# sc-136548), TNF receptor 1 (TNFR1; Cat#sc-9436), histone deacetylase 1 (HDAC1; Cat# sc-81598), HDAC3 (Cat# sc-376957), calnexin (Cat# sc-46669), lamin B1 (Cat# sc-374015), and flotillin-1 (Cat# 74566) were obtained from Santa Cruz Biotechnology.

    Techniques: Knockdown, Transfection, Control, Cell Culture, Incubation, Western Blot